Utskrift från Malmö högskolas webbplats www.mah.se

Water – a crucial factor in regulating biomembrane permeability

Contact person: Johan Engblom
Responsible: Johan Engblom
Co-workers: Sebastian Björklund, Emma Sparr (Lunds universitet) and Krister Thuresson (Delta of Sweden AB)
Partner: Lars Wadsö (Lunds universitet), Daniel Topgaard (Lunds universitet), Tautgirdas Ruzgas (MAH), Cathrine Albér (MAH), Vitaly Kocherbitov (MAH), Marie lodén (Eviderm Institute AB), Robert Cockery (YKI AB), Ulf Åkerström (ACO Hud Nordic AB)
Funding: FLÄK (Forskarskolan för läkemedelsvetenskap, Lunds universitet)
Timeframe: 2008-01-01 -- 2013-12-31
Research profile: Biologiska gränsytor
Research programme: Biofilms Research Center for Biointerfaces
Faculty/Department: Faculty of Health and Society, The Department of Biomedical Science
Subject: Naturvetenskap
Webpage: http://www.mah.se/biofilms

The skin is a very attractive route for drug delivery. A prerequisite for rationale design of new transdermal delivery principles is a solid understanding of mechanisms governing transdermal uptake. The aim of the present project is to explore basic mechanisms for drug permeation in the skin. The hypothesis is that permeability of the skin is to a large extent determined by co-variation in the physicochemical properties of the permeant and the gradient in water activity over stratum corneum. The specific goals are i) To explore how the water activity in a formulation can be used to regulate drug permeation in skin, ii) To investigate how the water gradient depends on the degree of occlusion and changes in skin temperature, iii) To investigate the co-variation in the physicochemical properties of alternative NSAID’s (non-steroidal anti-inflammatory drugs, as relevant model drug substances) and the gradient in water activity over skin for drug permeability in vitro, iv) To develop a theoretical model to support the interpretation of the experimental studies of skin permeation in the presence of gradients in water activity or temperature, and v) To investigate the IVIVC (in vitro-in vivo correlation) with formulations containing the same NSAID’s.

Publications: 

1. Sebastian Björklund, Johan Engblom, Krister Thuresson, and Emma Sparr; “A water gradient can be used to regulate drug transport across skin”; Journal of Controlled Release, 2010, vol 143, pp 191-200

2. Emma Sparr, Sebastian Björklund, Johan Engblom and Krister Thuresson; “A water gradient can be used to regulate drug transport across skin - A responding membrane”; Biophysical Journal, 2010, vol 98, issue 3, supplement 1, pp 627a

3. Sebastian Björklund and Lars Wadsö; “A calorimetric method to determine water activity”; Review of Scientific Instruments, 2011, vol 82, pp 114903-1-8 

4. Sebastian Björklund, Agnieszka Nowacka, Joke A. Bouwstra, Emma Sparr, and Daniel Topgaard; “Characterization of stratum corneum molecular dynamics by natural-abundance 13C solid-state NMR”; PLOS ONE, 2013, vol 8, e61889, pp 1-11

5. Sebastian Björklund, Johan Engblom, Krister Thuresson, and Emma Sparr; “Glycerol and urea can be used to increase skin permeability in reduced hydration conditions”; European Journal of Pharmaceutical Sciences, 2013, 50, 638–645

6. Sebastian Björklund, Tautgirdaz Ruzgas, Agnieszka Nowacka, Ihab Dahi, Daniel Topgaard, Emma Sparr, and Johan Engblom; “Skin membrane electrical impedance properties under the influence of a varying water gradient”; Biophysical Journal, 2013, vol 104, pp 2639-2650

7. Sebastian Björklund, Jenny Andersson, Dat Pham, Agnieszka Nowacka, Daniel Topgaard, and Emma Sparr; “Stratum corneum molecular mobility in the presence of natural moisturizers”; Soft Matter, 2014, 10, 4535-4546

Senast uppdaterad av Magnus Jando